EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.75 | Mus musculus | Q8R2H9 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.75 | MC3T3-E1 cell | - |
Mus musculus | - |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.75 | physiological function | cell line MC3T3-E1 clone 14 cells express high levels of Phospho1 and low levels of tissue-nonspecific alkalinephosphatase TNAP and they mineralize their matrix strongly. Clone 24 cells express high levels of TNAP and low levels of Phospho1 and mineralize their matrix poorly. Lentiviral Phospho1 overexpression in clone 24 cells results in higher Phospho1 and TNAP protein expression and increased levels of matrix mineralization. specific inhibition of Phospho1 individually reduces mineralization levels of Phospho1 overexpressing C24 cells, whereas the simultaneous addition of inhibitors of both Phospho1 and TNAP essentially abolishes matrix mineralization | Mus musculus |